Skip to main content

Table 6 Genotyping strategy to confirm insertion of the donor constructs

From: Whole genome sequencing facilitates intragenic variant interpretation following modifier screening in C. elegans

Strain

Forward (5′➔3′)

Reverse (5′➔3′)

Length

Annealing Tm

RE

MTG309

ACGACAGAGATCGAGGGAA

ACCGGAGGAGGATGTGAA

777 bp

61 °C

BbvI

MTG320

ACGACAGAGATCGAGGGAA

ACCGGAGGAGGATGTGAA

777 bp

61 °C

Hpy166II

MTG329

AGATGGTTGCTGTGACGATAAG

GGTACTCGATCAGTTCGCATAAA

792 bp

61 °C

BsrDI

MTG398

CCACTCTTTGTCCCACTCTAAA

CTCTTAATCGCCACCTTCTCTC

575 bp

52.5 °C

DdeI

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us