Fig. 1
From: Baiting out a full length sequence from unmapped RNA-seq data
Schematic overview of this strategy. Model read is selected from the screened unmapped reads. 5′ end SRTP (5′ end specific reverse transcription primer, green single strand) and 3′ end SRTP (3′ end specific reverse transcription primer, red single strand) are designed along the sequence of model read RNA and used to synthesize the first strand cDNAs. After the second strand cDNAs are synthesized and sequenced, the reads are mapped to reference genome and used to estimate the full length sequence. The clipart of Hiseq 2500 depicted in Fig. 1. is downloaded from Illumina official site (https://www.illumina.com/systems/sequencing-platforms/hiseq-2500.html) with the authorization