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Fig. 3 | BMC Genomics

Fig. 3

From: Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping

Fig. 3

UPIP-qPCR possessed wide applicability in identifying all kinds of variations in SNPs. UPIP-qPCR presented specific amplification signals and typical S-type curves in the genotype detection of 5 different SNPs with all kinds of variations except G-A and C-T, including interchanges between A-C, A-T, G-C, G-T,, and base InDels mutation (a). All three different genotypes of these SNPs were clustered in the scatter diagrams, and the results of genotyping were correct compared to Sanger sequencing(b). In each scatter diagram, WT-, heterogeneous-, and mutant-genotypes were represented by yellow dots, green triangles and blue squares, respectively. Each sample was detected with three duplicates at one experiment, and the experiments were repeated more than three times

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