deltaG cut-off | Primer names | Primer_sequences | Chr* | Length | Left_site deltaG△ | Right_site deltaG■ |
---|---|---|---|---|---|---|
<−13 k cal/mol | T13k_1 | GGCCTGGCTCTCTATACCCC | 6 | 325 | − 13,223 | −15,028 |
T13k_2 | ACGTTGCTCCCGCTCACC | 16 | 945 | −14,693 | − 14,693 | |
T13k_3 | AAGACCACGAGGCACAGC | 20 | 1778 | −13,580 | − 13,372 | |
T13k_4 | CCACAGCATCGTGACCCTG | 2 | 1453 | −15,972 | −15,972 | |
T13k_5 | AGCAAGGAAGTGGACAGGTC | 8 | 572 | −13,149 | −13,149 | |
T13k_6 | GTGTAGGAGCTGCGCCTC | 5 | 503 | −13,483 | −13,420 | |
T13k_7 | ATCTGCAGCAGTTTGGAGCC | 6 | 742 | −13,454 | −13,454 | |
T13k_8 | TCTCCACGATGCCGGCTG | 9 | 1402 | −13,003 | −14,414 | |
T13k_9 | GTTTCTTCTCCATCGCGGGG | 17 | 1130 | −13,847 | −13,573 | |
T13k_10 | CTCCATGGCTGATCTCCCCT | 16 | 1736 | −13,195 | −13,195 | |
−12 ~ − 13 k cal/mol | T12k_1 | CAGATCACCTACAGCGCCA | 11 | 1635 | −12,176 | −12,176 |
T12k_2 | TAACCCCCATAGCCCTCAAC | 1 | 1645 | −12,460 | − 12,621 | |
T12k_3 | CATATGGCCGTGTCTGGGG | 2 | 239 | −12,572 | −12,021 | |
T12k_4 | CCAGCCCAGCATTCTCCAG | 19 | 1864 | −14,841 | −12,519 | |
T12k_5 | GCAGCAGGGATTGGATGGC | 15 | 1776 | −12,218 | −12,218 | |
T12k_6 | GCAGGGATTGGATGGCCC | 11 | 855 | −12,436 | −12,115 | |
T12k_7 | CATCTTCGATTTGAGTGCCCC | 12 | 1868 | −12,150 | −12,150 | |
T12k_8 | TTCTTGAAGAGGACGGTGCC | 2 | 1298 | −12,580 | −12,380 | |
T12k_9 | TCTGAGCATTTGGGCTTGC | 3 | 984 | −12,562 | −12,671 | |
T12k_10 | CAAGATGTCGGGGAGTGGCC | 17 | 1502 | −12,976 | −12,900 | |
−11 ~ − 12 k cal/mol | T11k_1 | GGCTAAGGGGGTACACTTCA | x | 657 | −11,112 | −11,112 |
T11k_2 | CTTGGAAGTGGACGTAGGTGT | x | 802 | −11,333 | −11,333 | |
T11k_3 | GGTGTCTGTCCCGGCCAG | 4 | 529 | −13,905 | − 11,348 | |
T11k_4 | CATTTACAATCCAGCCAAATCACT | 7 | 1475 | −11,879 | −11,879 | |
T11k_5 | GCTCCTGGGGCATAGGGATG | 3 | 335 | −11,911 | −11,911 | |
T11k_6 | CAGGGATTGGATGGCCCC | 11 | 516 | −11,193 | −11,193 | |
T11k_7 | CTGGCTTGGCACTGGTCT | 15 | 916 | −11,926 | −11,322 | |
T11k_8 | AGCCCTGTGTGGTTCCAAG | 6 | 916 | −11,194 | −11,175 | |
T11k_9 | CTCACTCCATCCTGGACGT | 2 | 452 | −11,110 | −11,110 | |
T11k_10 | GCTCACTCCATCCTGGACGT | 2 | 452 | −11,868 | −11,868 | |
−10 ~ − 11 k cal/mol | T10k_1 | CCTCCTTGTGCCCTAGAGTT | 14 | 185 | −10,271 | −10,271 |
T10k_2 | cctttctgttgcctGTGGTAAG | 5 | 161 | −10,543 | −10,224 | |
T10k_3 | GCTTTTCCTCATGGGGCAAA | 4 | 447 | −10,575 | −10,809 | |
T10k_4 | CCAGGACAAGGCCTCATCC | 4 | 325 | − 10,242 | − 10,853 | |
T10k_5 | TTCCATGGCAGACTTGTAAGGA | 14 | 401 | −10,934 | −10,934 | |
T10k_6 | CAGTGTGGTAAAAACGTCAGGATG | 2 | 464 | −10,167 | −10,167 | |
T10k_7 | AGTTGCAGAACCTTGCCCT | 14 | 375 | −10,812 | −10,350 | |
T10k_8 | TCGAGACGGGGGTAAAGGAG | X | 147 | −10,799 | −10,073 | |
T10k_9 | AGGAATTCATGCCTTTGGGACA | 6 | 191 | −10,203 | −10,678 | |
T10k_10 | CTGCTCCATGGCTGATCTCC | 4 | 316 | −10,235 | −10,809 | |
−9 ~ − 10 k cal/mol | T9k_1 | ATGGAGGCTGGATAGGAGGT | 1 | 162 | − 9516 | − 9084 |
T9k_2 | GCCTCCTTGTGCCCTAGAGT | 14 | 187 | − 9598 | −9598 | |
T9k_3 | GGGTCTTGGCAAGTGGTACTG | 6 | 394 | − 9068 | − 9307 | |
T9k_4 | GCTCCTGGGGCATAGGGAT | 3 | 337 | − 9737 | −9737 | |
T9k_5 | GATTTCAGGGTGGTCACAGT | 2 | 419 | − 9299 | − 9050 | |
T9k_6 | ACGCTGCTATGGTTCTACAG | 17 | 276 | − 9320 | − 9506 | |
T9k_7 | GCAGCAGGGACTCACCATTG | 2 | 409 | − 9396 | − 9861 | |
T9k_8 | ATTCAGCGTGATGTGTAATGAGG | 9 | 428 | − 9135 | − 9334 | |
T9k_9 | AGAGGCTCCTGCAATTGACAG | 9 | 302 | − 9394 | − 9330 | |
T9k_10 | TCAGACCAGTGCTACCTGAGAG | 9 | 202 | − 9438 | − 9577 |