Fig. 4
qRT-PCR validation of the miRNA expression levels detected by small RNA sequencing. A. Correlations of miRNA expression profiles between small RNA sequencing and qRT-PCR. Log2(ratios) of qRT-PCR and small RNA-Seq were used to draw the scatter diagram. The trend line, regression equation and correlation coefficient were generated automatically in EXCEL. B. qRT-PCR analysis of miR156, miR166, miR398 and miR396 in P. nigrum. PnHis3 was used as an internal control. Data are the means ± SD from 3 biological replicates