Table 3 Primers used for qPCR of β-actin and sex-biased genes
Name | Primer | Sequence (5′˗3′) | Length (bp) |
|---|---|---|---|
β-actin | Forward | CAGATGTGGATCAGCAAGCA | 245 |
Reverse | GCTGAAGTTGTTGGGCATTT | ||
gnrh1 | Forward | GCTGGTCGGGAGTCTGATGT | 155 |
Reverse | AACCCAGAAGAGCGGAGGA | ||
cyp19b | Forward | AACAAGTACGGCAGCCTGG | 153 |
Reverse | TCCCTCCATCCCGATACACT | ||
cyp1a1 | Forward | ATGGCACCGAGGTCAACAA | 119 |
Reverse | CAGGATTGCCAGGAAGAGGTA | ||
prph | Forward | AAGCCATAGGAAAGGAGAGGG | 137 |
Reverse | GCGGAAGGCAATCAGGTTA | ||
nart1 | Forward | TTCCCACAATAACCAGCATCA | 147 |
Reverse | CACGCTTACACTTTCAGCAACA | ||
arntl1a | Forward | TCCTGTTTGTGGTCGGTTGT | 181 |
Reverse | CTCTCTCGTGGGGCTGTATCT | ||
bhlbe | Forward | GCGACGGCAAAGATAAAGATAC | 200 |
Reverse | CTGTCCCACGCTGCTTATTC | ||
nr1d2 | Forward | CGCCCACATCAACAAGGA | 187 |
Reverse | ATGTGCGTAGGTGGGAGTGT | ||
per1b | Forward | CACCCTCAACGCACTCAAA | 175 |
Reverse | GTCGGTGTTTTTCAGGGTGTA | ||
per3 | Forward | ACAATGGTTCCAGCGGTTAT | 109 |
Reverse | TGCGAGTCCTCCCACAGA | ||
cipc | Forward | ACAGGGTCAAAGGAAGGGTG | 105 |
Reverse | GTTGGTGATGCTGATGCTTGT | ||
cry1 | Forward | AGGCGGGTGTAGAGGTCATT | 110 |
Reverse | GGTCTGGAAACGCTTGTAGGT | ||
ciart | Forward | CGCTCCCTCCAAGATTCCT | 145 |
Reverse | TGAGACGAGGGCACTTGTAGAG |