Fig. 1

AL6 is critically involved in jasmonate-inhibited hypocotyl elongation during skotomorphogenesis. A, Phenotype of 5-d-old Col-0 (wild type, WT) and al6 seedlings on mock (ES) medium, or media supplemented with 50 μM JA (+JA), 2.5 μM Pi (low Pi), or 2.5 μM Pi + 50 μM JA (low Pi+JA) in darkness. B, Quantification of hypocotyl length. Three independent experiments with n ≥ 60 were performed. Error bars represent SE. C, D, Confocal laser scanning (C) and cryogenic scanning electron (D) micrographs of hypocotyl epidermal cells from wild-type and al6 seedlings. Bar = 20 μm. E, Hypocotyl cell length. Error bars represent SE, n ≥ 30. F, Quantification of JA levels. JA concentration was quantified by liquid chromatography-tandem mass spectrometry after solid-phase extraction of methanolic extracts. Data are from three biological replicates and expressed as picomoles per milligram of fresh weight (FW). Letters above bars indicate significant differences (P < 0.01) as determined by two-way ANOVA with Tukey test using multiple comparison between cell means regardless of row and columns. GraphPad Prism 8.0 was used to generate graphs and to conduct statistical analysis