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Fig. 3 | BMC Genomics

Fig. 3

From: Alternative splicing level related to intron size and organism complexity

Fig. 3

ASP/L does not correlate with intron size among genomes. (A) Distribution of Spearman’s ρ and P values of the correlations between ASP/L and CTN plus the three intron size-related statistics for the three sets of species. The correlation was calculated using all species (blue) or fewer species (pink). For the ThirdSpeciesSet, the fewer species calculation excluded Pst. The blue boxes represent the partial correlation produced by removing the effect of CTN or mean intron size. (B) The scatter plots depict the correlation between three intron size-related statistics, mean intron size (left), coding sequence (CDS) density (middle), and intron density (right), and two species-level complexity proxies, organism complexity (CTN; the blue points and the y-axis on the left) and alternative splicing level (ASL2; the red points and the y-axis on the right) from Chen’s data [3]. (C) Same analysis as in (B) but only using species from the FirstSpeciesSet. The gray data points represent the three species that were excluded because of the outlier relationship between mean intron size and CTN. The gray line represents the linear regression line that was fitted using all of the species. The three excluded species were Ame, Pvi, and Mun. ASL was calculated using the LeafCutter tool and a Bin500 gene number. (D) Same analysis as in (B) but only using species from the SecondSpeciesSet. The three excluded species were Ofa, Hvu, and Tca. The Spearman’s ρ in (BD) was illustrated in Additional file 11: Figure S4. (E) Same analysis as in (B) but using only species from the ThirdSpeciesSet with fixed organism complexity

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