Characterisation of novel endogenous geminiviral elements in macadamia

BMC Genomics

Table 2 Primers for flanking regions and overlapping fragments of macadamia associated geminiviral genome

Accession No.	Primer name	Sequence	Annealing temperature (°C)	Target
UZVR01000870	USFR_F	CACCAGCTCAAAGTATCTCC	52 (USFP)^a	Up and downstream flanking region
	USFR_R	ATGTAAGCCTTCCCTCTCGA	52 (USFP)^a
	DSFR_F1	GGTTACCATCTGACATGCTCAAGGCT	61 (DSFP)^a
	DSFR_R1	TGCTGGAACTGTGGTCTGATTTCAGTCC	61 (DSFP)^a
	UZVR1_F3	GGTCGTACTTTCGATCTCTGATCTGAGG	56	Macadamia associated EGE assemblage 1
	UZVR1_R3	GGGTGGAATCCCAAGTATTGTCTTATGC	56
	UZVR_2F	CCACATTCTGTATCGTCCAT	53
	UZVR_2R	TATCCTAAATGTCCACTTCC	53
	UZVR_3F	CTCCTTTGGAAGTGGACATTTA	55
	UZVR_3R	ATAAGAGAGTCCTTATCCTCC	55
	UZVR_4F	GTTGCGGTTATTGAGTCGAA	54
	UZVR_4R	GAGCATCAAAGTCCAGTATC	54
	UZVR_5F	GTGACACCGACATTCGAACT	54
	UZVR_5R	AGTTGCCGCAGAAGTACACG	54
	UZVR_6F	TACTTCTGCGGCAACTCTGATG	53
	UZVR_6R	GTCGAGTCTGTCACTCCTTC	53
FLKO01046132	FLKO_1F	TTAGGGCATGGGTGTAAAATC	60	Macadamia associated EGE assemblage 2
	FLKO_1R	AAGAACCACAATGATGACTG	60
	FLKO_2F	GATAATCCTAGACCTGGATTGC	56
	FLKO_2R	ATATTCCATATGCCGCGTTC	56
	FLKO_3F	CTGAACAGAGCTTGAATCAG	55
	FLKO_3R	GAGAGTCCTTATCCTCCATG	55

ISSN: 1471-2164