Fig. 2

Colocalization of early and late endosomal markers with the A. flavus conidia. RCB2280 cells were infected with A. flavus (CI1698) swollen spores for 4 h and stained for endosomal markers. (A) A. flavus in early endosomes (CD71) (B) A. flavus in late endosomes (LAMP1). The endosomal markers (Red) were detected using dylight 550 conjugated goat anti-mouse secondary antibody. From left to right, panels show bright field, fluorescence image of the blue channel (DAPI), fluorescence image of red channel (endosomes), merged overlay of all fluorescent channels, White arrow shows the germinated conidia inside the early and late endosomes