Fig. 1

A Triangle plot of significantly (DESeq2: p < 0.1) DEGs (black circles) and non-DEGs (grey dots). Position denotes relative proportion of total expression between the three conditions so that genes upregulated in fed amphioxus are located towards the ‘Fed’ point of the triangle. B PCA plot with first and second primary components of variance of significantly DEGs (n = 795) separates the three treatment groups (shapes), without separating the two trials (colour). Each of the four individuals in a treatment group is labelled A, B, C, or D. C Number of genes and number of isotigs corresponding to that gene (in parentheses) significantly up- or down- regulated in three comparisons. Up-regulated from fed to fasted means significantly more reads were detected in the fasted amphioxus than in fed for that isotig. For some genes, some isotigs were up-regulated while others were down-regulated, hence a lower total number of genes than the sum of each direction. D Number of the significantly up- or down-regulated genes assigned to GO biological processes by WebGestalt analysis in the three comparisons