Table 4 Top 10 up-regulated genes differentially expressed between the bark and needles at T0 (before treatment) for each plant part. The table also shows the ID of the genes assigned in this study for ease of identification in the tables, Scion transcripts code, predicted gene name and function
Part | ID | Scion transcript code | Predicted gene name | Predicted gene function |
|---|---|---|---|---|
Bark | B1 | NZPradTrx054097_C01 | Homeobox transcription factor KN3 | Central regulators of meristem cell identity (Guillet-Claude et al. 2004) |
B2 | NZPradTrx073079_C03 | Transporter, putative | Sugar transport (Weig et al. 1994) | |
B3 | NZPradTrx087709_C01 | Homeobox transcription factor KN1 | Central regulators of meristem cell identity (Namroud et al. 2010) | |
B4 | NZPradTrx055579_C01 | Mini zinc finger 1 | Regulates several development aspects, including photomorphogenesis, apical dominance, longevity, flower morphology and fertility, as well as root and stem elongation (https://www.uniprot.org/uniprot/Q9CA51) | |
B5 | NZPradTrx048496_C01 | Plastid phosphate translocator | Involved in the exchange of metabolites and inorganic phosphate between stroma and cytosol (Bockwoldt et al. 2019) | |
B6 | NZPradTrx101882_C01 | Auxin-induced protein 5NG4, putative | Transmembrane transporter activity especially during root formation (Busov et al. 2004) | |
B7 | NZPradTrx103825_C01 NZPradTrx103825_C04 | PREDICTED: GDSL esterase/lipase At5g03610-like | Lipid catabolic process (https://www.uniprot.org/uniprot/Q9LZS7) | |
B8 | NZPradTrx184572_C01 | G1-like protein | Polymerizes the backbones of non-cellulosic polysaccharides (hemicelluloses) of plant cell wall | |
B9 | NZPradTrx055645_C01 | PREDICTED: squalene monooxygenase-like | Converts squalene into oxidosqualene, the precursor of all known angiosperm cyclic triterpenoids (Rasbery et al. 2007) | |
NZPradTrx096935_C03 | ||||
B10 | NZPradTrx093053_C01 | Ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit | Catalyses carboxylation of RuBP in the first step of the Calvin cycle of photosynthesis (Tabita 1999) | |
Needles | N1 | NZPradTrx115678_C04 | Anthocyanidin reductase | Involved in the biosynthesis of proanthocyanidins (Zhu et al. 2015) |
NZPradTrx115678_C05 | ||||
N2 | NZPradTrx090889_C01 | Cytochrome P450 CYPA2 | Oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen https://www.uniprot.org/uniprot/A9F9S4 | |
N3 | NZPradTrx114954_C01 | Glucosyltransferase | Transfer of glucose (Chen et al. 2016) | |
NZPradTrx086877_C02 | ||||
N4 | NZPradTrx088783_C01 | Glucose-1-phosphate adenylyltransferase, putative | Involved in the pathway starch biosynthesis (https://www.uniprot.org/uniprot/Q688T8) | |
N5 | NZPradTrx086324_C01 | PREDICTED: LOB domain-containing protein 1-like | Involved in the repression of the homeobox gene BP | |
N6 | NZPradTrx065580_C01 | Catalase | Crucial antioxidant enzymes that mitigates oxidative stress to a considerable extent by destroying cellular hydrogen peroxide to produce water and oxygen (Nandi et al. 2019) | |
N7 | NZPradTrx049683_C01 | Photosystem II core complex proteins psbY2C chloroplast precursor | Multi-component pigment-protein complex responsible for water splitting, oxygen evolution, and plastoquinone reduction (Lu 2016) | |
N8 | NZPradTrx097448_C02 | ribonucleoprotein, chloroplast, putative | Involved in chloroplast RNA processing (Tillich et al. 2009) | |
N9 | NZPradTrx119685_C01 | SOUL heme-binding protein | Plays an active role in primary plant metabolic pathways as well as in stress signalling (Shanmugabalaji et al. 2020) | |
N10 | NZPradTrx184701_C01 | chloroplast ribosomal protein S1 | Involvement in translation initiation via positioning of initiation mRNA–protein complexes (mRNPs), and the potential involvement of these unique domains in the processivity of chloroplast translation (Manuell et al. 2007) |