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Table 2 Summary of the RNA-seq data

From: Transcriptional analysis of Bemisia tabaci MEAM1 cryptic species under the selection pressure of neonicotinoids imidacloprid, acetamiprid and thiamethoxam

Sample

Raw reads

Clean reads

Clean bases (Gb)

Q20 (%)

Q30 (%)

GC (%)

Total reads

Total map

Unique map

SUS_A

48,504,690

48,490,522

7.27

95.86

90.03

40.07

48,490,522

41,725,916 (86.05%)

41,106,490 (84.77%)

SUS_B

50,196,366

50,181,356

7.53

96.22

90.68

41

50,181,356

43,847,577 (87.38%)

43,175,889 (86.04%)

SUS_C

53,504,154

53,487,908

8.02

96.15

90.6

40.3

53,487,908

46,166,534 (86.31%)

45,443,847 (84.96%)

IMI_A

42,456,440

42,443,694

6.37

96.3

90.88

40.97

42,443,694

37,056,407 (87.31%)

36,520,464 (86.04%)

IMI_B

63,185,144

63,166,110

9.47

96.14

90.42

43.41

63,166,110

56,409,149 (89.3%)

55,417,093 (87.73%)

IMI_C

47,763,844

47,749,700

7.16

96.2

90.74

40.23

47,749,700

41,246,966 (86.38%)

40,629,937 (85.09%)

ACE_A

55,560,384

55,543,710

8.33

96.15

90.59

40.52

55,543,710

47,371,626 (85.29%)

46,617,991 (83.93%)

ACE_B

48,844,738

48,830,222

7.32

96.12

90.51

40.18

48,830,222

41,837,768 (85.68%)

41,197,173 (84.37%)

ACE_C

44,189,186

44,175,744

6.63

96.03

90.38

40.18

44,175,744

37,349,722 (84.55%)

36,757,531 (83.21%)

THI_A

48,398,214

48,383,968

7.26

95.81

89.64

42.38

48,383,968

41,554,580 (85.89%)

40,870,267 (84.47%)

THI_B

53,127,326

53,111,186

7.97

96.08

90.53

40.09

53,111,186

44,531,359 (83.85%)

43,839,726 (82.54%)

THI_C

50,190,878

50,176,074

7.53

96.16

90.59

41.02

50,176,074

41,993,835 (83.69%)

41,330,531 (82.37%)

  1. SUS_A, B, and C: three repeats from susceptible MEAM1 cryptic species raised under control conditions without contact of any pesticide; IMI_A, B and C: three repeats from susceptible MEAM1 cryptic species selected continuously by imidaclorprid for 10 generations; ACE_A, B and C: three repeats from susceptible MEAM1 cryptic species selected continuously by acetamiprid for 10 generations; THI_A, B and C: three repeats from susceptible MEAM1 cryptic species selected continuously by thiamethoxam for 10 generations; Q20: the percentage of bases with a Phred value > 20; Q30: the percentage of bases with a Phred value > 30. Total reads: Number of clean reads after quality control; Total map: Number and percentage of clean reads mapped with reference genome; Unique map: Number and percentage of clean reads mapped with unique locus of reference genome