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Table 6 Validation of differentially expressed genes (DEG) by quantitative PCR

From: Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs

  

RNA-seq

qPCR

DEG between

Gene

FCa

FCa

p-value

SCD genotypes (TT vs CC)

SCD

2.15

3.00

0.003

diets in SCD_TT pigs (TT_VA+ vs TT_VA-)

ABCA1

1.85

1.41

n.s.

FGF10

5.30

1.95

0.03

PPARA

0.63

0.78

0.05

diets in SCD_CC pigs (CC_VA+ vs CC_VA-)

TNMD

131.44

253.75

0.04

OTOR

33.01

51.30

0.02

CILP2

34.57

14.21

0.03

SCD genotypes under the VA+ diet (TT_VA+ vs CC_VA+)

MTTP

2.63

1.97

n.s.

FGF10

6.55

2.08

0.03

SCD

2.84

4.05

0.03

  1. a FC, fold-change ratio between groups as indicated in the first column