Fig. 6

Tissue- and Injury-related changes in CpG methylation across the TSS (-2 kb – +2 kb) for all genes in the genome (N = 45,099 gene models), correlated with differential gene expression. Top and bottom panels illustrate average density and heatmaps of CpG methylation across the TSS for genes in each differential expression grouping. Whether a gene fell into the RNA Up, RNA Down, or no significant change category was determined by RNA-seq from RNA samples previously collected [11] from the same animals analyzed here by WGBS. Comparisons indicate changes between the first vs. second conditions (e.g., negative differential methylation and RNA Up indicate hypo-methylation and significantly increased RNA expression, respectively, in the first vs. the second condition, etc.). Injury vs. control heatmaps for non-regenerative CNS (frog hindbrain) resembled those generated by comparisons between regenerative vs. non-regenerative CNS (tadpole and frog eye vs. frog hindbrain, respectively) and the opposite of what was seen with CNS injury in the two regenerative CNS regions (i.e., tadpole SCI hindbrain and frog ONC eye). x-axis units, as in Fig. 4; y-axis units, Δ5mC within a 50 bp bin x 1 million/total number of Cs