Fig. 4From: Combined assembly of long and short sequencing reads improve the efficiency of exploring the soil metagenomeGene functional annotation determined using the COG (cluster of orthologous group) and KEGG (Kyoto Encyclopedia of Genes and Genomes) databases. The numbers of clusters and Ko-ids obtained by each method were plotted collectively (A and B). Pathways predicted by the three sequencing/assembly methods and the number of pathways in the KEGG [26,27,28] pathway database (C), and the number of genes corresponding to pathways predicted by the three sequencing/assembly methods and their proportion in each predicted gene (D). In the x-axis of panel C, “All” indicates the average number of pathways predicted in the DNA extracted from the high, medium, and low altitude soil samples. “DB” refers to the KEGG Pathway Database (url: https://www.genome.jp/kegg/pathway.html#environmental). The detailed function classes in Fig. 4A are described in supplementary materials Table S4 and the detail metabolic pathways were predicted using ipath 3 (url: https://pathways.embl.de/ [29]) and are shown in Fig. S5. The curves in panels A are arranged in descending order of PI dataBack to article page