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Fig. 1 | BMC Genomics

Fig. 1

From: Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes

Fig. 1

Comparison of Sequin spike-in sensitivity of detection between ONT (long read) and short read sequencing (Illumina paired-end, SRS) sequencing for Sequin synthetic spike-ins MixA (A) and MixB (B). Labelled sequin (red point) in each plot is at ONT limit of quantification (LOQ) in each mix; mix A 0.059 attomol/μl and mix B 0.27 attomol/μl. Plot C shows correlation of ONT differential isoform observed vs observed expression (log2 fold change + 1) of synthetic Sequin spike-ins of known concentration. Pearson correlation coefficient is displayed along with a linear regression trend line with standard error in pale grey

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BMC Genomics

ISSN: 1471-2164

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