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Table 1 Showing number of genes and transcripts differentially expressed between undifferentiated and differentiated cells, after multiple testing correction using ONT long read counts (FDR < 0.05). Bracketed numbers refer to the portion of total that are TALON-identified novel transcripts. U = undifferentiated and D = differentiated cells, with arrows displaying expression directionality

From: Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes

Metric

Count

Gene level

Transcript level (Talon)

Total features assessed

32,977

99,067 (1855)

Differentially Expressed

4239

5456 (197)

↑U ↓D (all > 0 log2FC)

2041

2390 (67)

↑D ↓U (all < 0 log2FC)

2198

3066 (130)