Table 2 Relative detection errors determined using the simulated dataset
Sample ID | No. of Reads Added in the SDa | No. of Unique Reads Detected as Chicken using 3MG method | Relative errors±STD | ||||||
|---|---|---|---|---|---|---|---|---|---|
Total No. of Reads | No. of Chicken Reads | R1b | R2 | R3 | R4 | R5 | Average | ||
SD01 | 30,000 | 3 (0.01%) | 30 | 31 | 29 | 31 | 29 | 30 | 900.00% ± 29.81% |
SD02 | 30,000 | 30 (0.1%) | 57 | 58 | 56 | 57 | 55 | 56.6 | 88.67% ± 3.40% |
SD03 | 30,000 | 300 (1%) | 323 | 322 | 316 | 322 | 321 | 320.8 | 6.93% ± 0.83% |
SD04 | 30,000 | 600 (2%) | 621 | 620 | 610 | 617 | 614 | 616.4 | 2.73% ± 0.67% |
SD05 | 30,000 | 900 (3%) | 916 | 918 | 909 | 912 | 909 | 912.8 | 1.42% ± 0.41% |
SD06 | 30,000 | 1200 (4%) | 1211 | 1213 | 1207 | 1207 | 1206 | 1208.8 | 0.73% ± 0.23% |
SD07 | 30,000 | 1500 (5%) | 1505 | 1507 | 1503 | 1502 | 1502 | 1503.8 | 0.25% ± 0.13% |
SD08 | 30,000 | 1800 (6%) | 1801 | 1804 | 1802 | 1801 | 1798 | 1801.2 | 0.07% ± 0.11% |
SD09 | 30,000 | 2100 (7%) | 2099 | 2100 | 2098 | 2094 | 2093 | 2096.8 | 0.15% ± 0.13% |
SD10 | 30,000 | 2400 (8%) | 2397 | 2396 | 2393 | 2391 | 2390 | 2393.4 | 0.27% ± 0.11% |
SD11 | 30,000 | 2700 (9%) | 2694 | 2691 | 2687 | 2686 | 2686 | 2688.8 | 0.41% ± 0.12% |
SD12 | 30,000 | 3000 (10%) | 2991 | 2986 | 2985 | 2975 | 2982 | 2983.8 | 0.54% ± 0.18% |
SD13 | 30,000 | 6000 (20%) | 5950 | 5945 | 5934 | 5934 | 5943 | 5941.2 | 0.98% ± 0.11% |
SD14 | 30,000 | 9000 (30%) | 8912 | 8898 | 8895 | 8898 | 8900 | 8900.6 | 1.10% ± 0.07 |
SD15 | 30,000 | 12,000 (40%) | 11,850 | 11,859 | 11,860 | 11,859 | 11,870 | 11,859.6 | 1.17% ± 0.05% |
SD16 | 30,000 | 15,000 (50%) | 14,812 | 14,818 | 14,817 | 14,824 | 14,825 | 14,819.2 | 1.21% ± 0.03% |
SD17 | 30,000 | 18,000 (60%) | 17,781 | 17,790 | 17,795 | 17,779 | 17,777 | 17,784.4 | 1.20% ± 0.04% |
SD18 | 30,000 | 21,000 (70%) | 20,752 | 20,753 | 20,757 | 20,741 | 20,738 | 20,748.2 | 1.20 ± 0.04% |
SD19 | 30,000 | 24,000 (80%) | 23,713 | 23,726 | 23,721 | 23,716 | 23,709 | 23,717 | 1.18% ± 0.02% |
SD20 | 30,000 | 27,000 (90%) | 26,671 | 26,684 | 26,684 | 26,686 | 26,678 | 26,680.6 | 1.18 ± 0.02% |
SD21 | 30,000 | 30,000 (100%) | 29,633 | 29,651 | 29,629 | 29,652 | 29,646 | 29,642.2 | 1.19% ± 0.03% |