Fig. 5From: Spatiotemporal expression profile of novel and known small RNAs throughout rice plant development focussing on seed tissuessiRNA-regulated genes in rice embryo and endosperm. A-B Overlap between siRNA24 loci (A) or siRNA21 loci (B) in embryo and endosperm, and protein-coding genes in their 1 kb upstream, gene body or 1 kb downstream regions. For both tissues, all coding genes were categorized based on their expression level in the corresponding tissue: high (FPKM > 10), medium (FPKM between 10 and 2), low (FPKM between 2 and 0) or not expressed (FPKM = 0). n indicates the number of protein-coding genes in each group. Permutation tests were conducted as described in Fig. 2. Data where the null distribution did not resemble a normal distribution are not shown. C FPKM density of expressed siRNA21 in all tissues and for siRNA21 overlapping expressed protein-coding genes in the endosperm (red dashed line). The box indicates the FPKM cut-off for the exceptionally highly expressed siren21 loci. D-E Number of predicted targets of siRNA24 in the embryo (D) and endosperm (E), overlapping with the promoter (1 kb upstream), the gene body or 1 kb downstream regions of coding genesBack to article page