Fig. 2

DEGs were compared between relatively higher expression and lower expression groups of PD-1/PD-L1 by analyzing the microarray database GSE135820. Heatmaps show DEGs that were related to PD-1 (A) or PD-L1 (B) in the two groups. Red indicates DEGs that were positively associated with PD-1/PD-L1, white indicates DEGs that were not related to PD-1/P-L1, and blue indicates DEGs that were negatively related to PD-1/P-L1. Volcano plot demonstrates different scattering of genes between high and low expression cluster groups of PD-1 or PD-L1. Genes (depicted in green or red) showed a fold change difference of > 0.5 and p < 0.05. Correlations of DEGs that were related to PD-1 (C) or PD-L1 (D) between two groups are shown in the correlation heatmap. Red indicates a positive correlation (r = 1), white indicates no correlation (r = 0), and green indicates a negative correlation (r = −1). DEGs, differentially expressed genes; PD-1, programmed death-1; PD-L1, programmed death ligand 1