From: Long range PCR-based deep sequencing for haplotype determination in mixed HCMV infections
pError | Template HCMV DNA | Input DNA | Reference length in kb | Number and average read length of sequencing reads | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
raw reads | after qtrim | after hu removal | % of reads < 500 bases | after ltrim | % HCMV mapping | map to reference | average coverage | |||||
0.01 | Merlin | amplicons | 15.5 | number | 92,128 | 63,604 | 30,632 | 18.7 | 24,909 | 98.26 | 24,475 | 6885 |
length | 4656 | 2820 | 3741 | 4547 | ||||||||
TB40-BAC4-luc | amplicons | 15.5 | number | 73,439 | 46,547 | 42,060 | 19.1 | 34,041 | 99.53 | 33,881 | 9062 | |
length | 6162 | 3590 | 3767 | 4601 | ||||||||
Merlin | non-enriched | 237 | number | 143,728 | 102,444 | 5166 | 13.9 | 4447 | 85.29 | 3793 | 45 | |
length | 4378 | 2722 | 2956 | 3397 | ||||||||
TB40-BAC4-luc | non-enriched | 235 | number | 96,966 | 72,804 | 9122 | 12.6 | 7975 | 93.63 | 7467 | 73 | |
length | 3583 | 2439 | 2417 | 2731 | ||||||||
0.001 | Merlin | amplicons | 15.5 | number | 92,128 | 50,031 | 22,247 | 36.4 | 14,154 | 97.76 | 13,837 | 2444 |
length | 4656 | 1631 | 1896 | 2843 | ||||||||
TB40-BAC4-luc | amplicons | 15.5 | number | 73,439 | 34,137 | 30,361 | 37.2 | 19,070 | 99.26 | 18,929 | 3118 | |
length | 6162 | 1814 | 1865 | 2828 | ||||||||
Merlin | non-enriched | 237 | number | 143,728 | 83,991 | 4028 | 27.2 | 2931 | 86.49 | 2535 | 25 | |
length | 4378 | 1849 | 1934 | 2566 | ||||||||
TB40-BAC4-luc | non-enriched | 235 | number | 96,966 | 60,539 | 7402 | 23.2 | 5688 | 94.57 | 5379 | 45 | |
length | 3583 | 1727 | 1751 | 2205 |