Table 4b Ratio estimation of dilution series of artificial mixtures
From: Long range PCR-based deep sequencing for haplotype determination in mixed HCMV infections
Artificial Mixture | HCMV-DNA | Aspired Input ratio | Estimated copies/ml | Copy input for PCR | Measured ratio2 |
|---|---|---|---|---|---|
1 | Merlin | 1 | 1,90E+ 05 1 | 1.90E+ 03 | 1.5 |
TB40-BAC4-luc | 99 | 3,90E+ 06 1 | 3.90E+ 04 | 98.5 | |
1:10 | Merlin | 1 | 1.90E+ 04 | 1.90E+ 02 | 1 |
TB40-BAC4-luc | 99 | 3.90E+ 05 | 3.90E+ 03 | 99 | |
1:100 | Merlin | 1 | 1.90E+ 03 | 1.90E+ 01 | 1.5 |
TB40-BAC4-luc | 99 | 3.90E+ 04 | 3.90E+ 02 | 98.5 | |
1:1000 | Merlin | 1 | 1.90E+ 02 | 1.90E+ 00 | 0 |
TB40-BAC4-luc | 99 | 3.90E+ 03 | 3.90E+ 01 | 100 |