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Table 5 Number and ratio of individual haplotype sequences with corresponding gN, gO, and UL146 genotypes

From: Long range PCR-based deep sequencing for haplotype determination in mixed HCMV infections

Sample

Haplotypes determined by long amplicon PacBio sequencing

Genotypes2 assigned by short amplicon Illumina sequencing

 

Target site

Seq ID

Reference sequence Acc.No. (% identity)1

Frequency of occurrence (%)

gN

gO

UL146

BAL2

F3

BAL2_F3_hap1

JX512202.1 (99.9)

58.7

3b

2a

 
  

BAL2_F3_hap2

KP745677.1 (99.1)

35.1

3b

2a

 
  

BAL2_F3_hap3

KJ361966.1 (99.3)

6.2

1

1a

 
 

F4

BAL2_F4_hap1

KP745638.1 (99.9)

59.2

  

13

  

BAL2_F4_hap2

KP745654.1 (98.6)

32.3

  

13

  

BAL2_F4_hap3

KT726941.2 (98.3)

8.5

  

12

BAL4

F3

BAL4_F3_hap1

KR534203.1 (99.9)

100

1

1a

 
     

3b

2a

 
 

F4

BAL4_F4_hap1

KU550090.1 (99.9)

100

  

14

       

13

BAL5

F3

BAL5_F3_hap1

KY490065.1 (99.9)

78.1

3b

2a

 
  

BAL5_F3_hap2

JX512208.1 (99.9)

14.1

2

2b

 
  

BAL5_F3_hap3

KY490066.1 (99.9)

7.8

3a

1b

 
 

F4

BAL5_F4_hap1

KR534198.1 (99.8)

75.1

  

6

  

BAL5_F4_hap2

KP745691.1 (99.8)

16.8

  

7

  

BAL5_F4_hap3

JX512208.1 (99.9)

8.1

  

11

BAL6

F3

BAL6_F3_hap1

KR534203.1 (99.9)

100

1

1a

 
     

3b

2a

 
 

F4

BAL6_F4_hap1

KY490086.1 (99.9)

85.6

  

2

  

BAL6_F4_hap2

KY490085.1 (97.1)

14.4

  

1

  1. 1) Sequence in NCBI Nucleotide database with highest similarity to haplotype sequence; 2) according to Suarez et al., 2019, doi: https://doi.org/10.1093/infdis/jiz208; BAL, bronchoalveolar lavage; Acc.No., Accession number