Table 2 Designed primer sequences with the respective annealing temperature and amplicon size for gene amplification
Target gene | Primer name | Sequence (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
12S rRNA | Tre12S-F | GTGCCAGCADYYGCGGTTA | 55 | 371 |
Tre12S-R | AGCAGCAYATHGACCTG | Â | Â | |
16S rRNA | CesTre16S-F | GTGYDAAGGTAGSATAAT | 56 | 379 |
CesTre16S-R | CCGGTYTYAACTCARCTCAT | Â | Â |