Fig. 1

Non-uniform localization of histone variants in T. gondii. Distribution of histone variants in the nucleus of tachyzoite forms. HFF cells were grown on glass slides and infected with tachyzoites for 24 h. Slides were stained with DAPI and antibodies against anti-H2AZ, anti-myc (for H2AX-myc and H2BZ-myc) or anti-HA (H3.3). Images were collected along a Z-series and processed for three-dimensional deconvolution using similar signal thresholds for each of the antibodies. The small extranuclear DAPI staining in some cells labels the genome of the apicoplast. The merged figures correspond to the overlay of DAPI (blue) and histone variants (green). The scale bars correspond to 5 μm