Fig. 4

Grain hardness of F₂ individuals of the cross between wild-type “Kitahonami” and the grain hardness mutant “30579”. a The histogram of grain hardness of F₂ individuals. b The results of PCR amplification of the indel markers of 5D (Pina-D1 and Pinb-D1), 2A, and 4B. W and G indicate the wild-type and the mutant, respectively. c Relationship between grain hardness and genotypes corresponding to 5D, 2A, and 4B deletions. Boxplots showing the effect of deletion mutations in comparison to wild-type on grain hardness. Dots in the boxplots indicate grain hardness for each sample. Genotypes of 22 soft grain and eight hard grain lines were evaluated based on the indel markers for 5D (Pina-D1 and Pinb-D1), 2A, 4B deletions. A plus sign ( +) indicates the absence of deletion, and a minus sign (–) indicates presence of deletion. Student's t-test was performed to test the statistical significance of the differences between the genotypes. Here, *** indicates P < 0.01 whereas ns: refers to nonsignificant. The full gel image of b is available in Additional file 3: Fig. S4