Fig. 7

Associations between PHS tolerance and the deletion on chromosome 3B in the F₂ population from a cross between Kitahonami (WT) and 28511 (PHS mutant). A A schematic diagram showing the location of the co-dominant markers designed to detect the deletion on chromosome 3B. A 712 bp PCR fragment is amplified by an “in-deletion” primer, and a “post-deletion” primer in wild-type Kitahonami (WT) whereas a 414 bp PCR fragment amplified by “pre-deletion” primer and “post-deletion” primer is expected in the PHS tolerance mutant 28511. Both fragments are amplified in heterozygous individuals in F₂ population. B Results showing the specificity of the co-dominant markers in the PCR amplification of alleles from WT, “28511”, and the Chinese Spring nulli-tetrasomic line N3BT3D in which chromosome 3B is replaced with chromosome 3D. C The seed dormancy test for the F₂ segregation population of Kitahonami (WT) × the mutant 28511. Boxplots of germination rate for each genotype in 3 days, 7 days, and 9 days are shown. The dots indicate germination rate for each sample. Where W, H, and M are lines carrying homozygous WT, heterozygous and homozygous mutant alleles, respectively. D The ABA sensitivity test for root elongation in WT and the mutant, where ns indicates nonsignificant difference based on Student’s t-test. The full gel image of B is available in Additional file 3: Fig. S5