Fig. 8

Relationship between PHS tolerance and presence/absence of the candidate genes on chromosome 3D. a Primer positions of Viviparous-B1 and the GRAS transcription factor genes designed to confirm the presence/absence of these genes in “Chinese Spring” (CS) deletion lines at chromosome 3B (Endo and Gill, 1996). b The two primer sets were designed to amplify two fragments for each gene. PCR amplifications of Viviparous-B1 and the GRAS transcription factor gene in wild-type “Kitahonami” (WT), the PHS-tolerant mutant “28511,” CS, the CS 3BL ditelosomic line (CS ditelo 3BL), and the CS nulli-tetrasomic line of nulli-3B tetra-3D (CS N3BT3D). The full gel image of b is available in Additional file 3: Fig. S6