Fig. 1
From: miR-486-5p expression is regulated by DNA methylation in osteosarcoma
miR-486-5p expression and methylation in osteosarcoma cell lines and patient samples. A. miR-486-5p expression level in normal bone (n = 6), cell lines (n = 17) and patient samples (n = 9) using qRT-PCR. The expression level was quantified for the groups of samples. The values are shown relative to mean expression of bone. B. DNA methylation and miRNA expression levels in normal bone samples (n = 4) and osteosarcoma cell lines (n = 19) using arrays. The expression level of miRNAs (log2) and DNA methylation level (Beta, probe cg00176210) were obtained using Agilent miRNA array v2 and Illumina Infinium Methylation27 BeadChip technology, respectively. Values are given as mean (SD) with whiskers from min to max. P-values * < 0.01 and ** < 0.0001