Fig. 2

Pangenome analysis of Brachyspira hyodysenteriae isolates of the predominant ST196. Total number of genes as well as bin-specific gene clusters (GCs) are indicated. A The two outmost layers represent both functional annotations derived from the COGs database and NCBI-PGAP. Known and unknown functions are in dark and light blue, respectively. The number of either known or unknown functions are indicated in brackets. Each blue-colored layer represents the genome of a B. hyodysenteriae isolate of ST196. The inner one represents the complete genome of B. hyodysenteriae isolate Bh743-7 used as reference genome. Genomes are organized by average full nucleotide identity (ANI values: 0.995 − 1) and minimum number of genomes in which a certain gene is present. Core (grey), soft core (yellow) and singletons (orange) bins are shown for all the genomes (n = 15). B Soft-core and singleton bins are represented in more detailed at the complete genome level (n = 15), but also for the plasmids (n = 13), independently