Micronutrient supplementation affects DNA methylation in male gonads with potential intergenerational epigenetic inheritance involving the embryonic development through glutamate receptor-associated genes

Table 4 The number of DMC, DMGs, enriched KEGG pathways and GO terms, and DEG:DMCs

	n^a	DMC^b	DMG^c	KEGG^d	GO^e	DEG:DMC^f
						Gonads	Liver	G&L
Gonads
L2:L1	3:3	27 433	9 774	20	107	0	21	20
L3:L1	3:3	26 995	9 647	12	95	26	59	69
Liver
L2:L1	3:3	25 420	9 370	16	125	2	20	18
L3:L1	3:3	26 587	9 512	19	153	26	72	80
G&L
L2:L1	6:6	3 786	1 981	1	13	0	2	2
L3:L1	6:6	3 716	1 977	2	25	7	16	21

^aNumber of samples represented as (# treatment group):(# control group). ^bNumber of DMCs defined by q-values < 0.01 and methylation rate differences > 25%. ^cNumber of DMGs defined as the genes with at least one DMC. ^dNumber of enriched KEGG pathways identified by ORA with adjusted p-values < 0.05 and the minimum gene counts of 20. ^eNumber of enriched GO terms by ORA with adjusted p-values < 0.001 and the minimum gene counts of 10. ^fNumber of DEGs that contain at least one DMC for gonads, liver and G&L (gonads and liver) datasets. Numbers in bold font are emphasised for those with matched datasets as gonads vs gonads, liver vs liver, and G&L vs G&L

ISSN: 1471-2164