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Fig. 1 | BMC Genomics

Fig. 1

From: Computational identification of clonal cells in single-cell CRISPR screens

Fig. 1

Multiplexed sgRNAs serve as a clonal barcode. A. Overview of single-cell CRISPR screen performed in this study (~ 20 k sgRNAs, ~ 50 k cells sequenced, ~ 30 sgRNAs per cell). B. Examples of clonal (left) and non-clonal (right) cells. Shown is the overlap of sgRNAs between two sequenced cells and the significance of this overlap (hypergeometric p-value). C. The proportion of clonal cells and non-clonal cells in the single-cell CRISPR screen dataset. 96% of sequenced cells have some degree of clonality, with at least one other cell sharing significant overlap of sgRNAs. D. We sampled 500 random cells from either clonal (left) or non-clonal (right) populations and then calculated p-values of sgRNA overlap for each pair of sampled cells. Shown is a heatmap of these p-values. E. Distribution of pairwise sgRNA overlap rate in 54 major clones. Given two cells, the sgRNA overlap rate is the number of shared sgRNAs (intersection) divided by the number of total sgRNAs (union). F. Pairwise sgRNA overlap rate for cells: in the same clone, between different clones, between clones and non-clones, and within non-clonal cells. Given two cells, the sgRNA overlap rate is the number of shared sgRNAs (intersection) divided by the number of total sgRNAs (union)

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