Table 4 Details of primer pairs for the chicken NUDT15 gene
Primer set | Primer sequence, 5′-3′ | Tm, °C | Product size, bp |
|---|---|---|---|
NUDT15-indel-2777-F | TGTGAGCTACACAGCTAGAACT | 61 | 113 |
NUDT15- indel-2777-R | ATTGGAGGGCTTGGCAATCA | Â | Â |
NUDT15- indel-1673-F | ACAGGAGCACATAAATCATAGCCT | 62 | 150 |
NUDT15- indel-1673-R | TGGCCAGTATTTTTAAGTGTGATGA | Â | Â |
qPCR-NUDT15-F | ACCTGGAGTTCGGGGAGAG | 60 | 145 |
qPCR-NUDT15-R | ATGAGCACGGTGACGTAGTG | Â | Â |
GAPDH-F | GAACATCATCCCAGCGTCCA | 55–65 | 132 |
GAPDH-R | CGGCAGGTCAGGTCAACAAC | Â | Â |
CCND1 -F | CAGAAGTGCGAAGAGGAAGT | 59 | 188 |
CCND1 -R | CTGATGGAGTTGTCGGTGTA | Â | Â |
CCNB2-F | CCTCTTCCACTTCACTTCT | 59 | 195 |
CCNB2-R | CTTTGTACCCCACTTATCA | Â | Â |
PCNA-F | AGCACCAAATCAGGAAAAG | 59 | 177 |
PCNA-R | GCACAGGAGATGACAACAG | Â | Â |