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Fig. 1 | BMC Genomics

Fig. 1

From: CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion

Fig. 1

Algorithmic Framework for CRISPR Cas13 Guide RNA Prediction. CRISPR Cas13 knockdown experiments, protein occupancy, and transcriptomic alignment data was gathered for consideration and analysis by the model. Feature lists were created through composition analysis and k-mer capture. The significance and contribution of each feature was estimated to create finalized possible lists of features. The final feature list for the model was generated through comparison of 3-fold and 5-fold cross-validation experiments. Model predictions were validated through direct comparison with performed experiments. The model used to predict sgRNA’s spanning all transcripts associated with 5000 genes. The results were collected and analyzed for any potential biological relevance for predictions

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