Fig. 8

Locomotor analysis of natterin-like depleted (KO) zebrafish larvae. The 144 h post-fertilization (hpf) KO or wild-type (WT) larvae were distributed in a 96-well plate (1 larva/well; 20 larvae per group) in 100 μL of medium and the locomotor activity represented by the distance moved was analyzed through the Zebrabox system (ViewPoint). Larvae were exposed to an acclimatization period of 20 min in the dark followed by 5 min of alternated 25 s light cycles (15% light stimulus) interspersed with 5 s dark cycles (0% light stimulus) to induce visual and neurological stimulation