Fig. 1

Detection of lysine 2-hydroxyisobutyrylation in F. graminearum. A SDS-PAGE gel stained with Coomassie blue was used as a loading control. Total proteins in F. graminearum treated with tebuconazole (TEC), carbendazim (BCM) or an equal volume of DMSO as control were loaded in each lane. B Western blotting analysis of the samples in (A) was performed with pan-2-hydroxyisobutyryllysine antibody. The bands corresponding to the samples in (A) were shown. C Schematic illustration of the proteomic analytical steps for mycelium collection, protein extraction, tryptic digestion, TMT labeling, affinity enrichment, LC–MS/MS and bioinformatics analysis