Fig. 2

Phylogenetic analysis and multiple alignment analysis of 40 EgWRKY proteins. A The phylogenetic tree was constructed with MEGA X using UPGMA method based on protein sequences of WRKY domains found in the 40 EgWRKYs which were aligned using Clustal W prior to phylogenetic tree construction using MEGA X based on UPGMA method using Jones-Taylor-Thronton (JTT) substitution model and partial deletion method with 1000 bootstrap value. B Multiple sequence alignment of conserved WRKY domain in 40 EgWRKY proteins was performed using Clustal W programme. Conserved WRKY motif and zinc-finger pattern are indicated within Group or Subgroups with dark grey represents 100% sequence identity. Groups I_N and I_C indicate the N-terminus and C-terminus of the WRKY domain of Group I EgWRKY protein