Fig. 7

Functional verification of FABP4 in GCs. A The cell viability after overexpression and interference of FABP4 in adult GCs. The value of control group (0) was set to 100%. B The relative expression levels of BAX, BCL2 and Caspase3 after overexpression and interference of FABP4 gene in adult GCs. C Detection of cell apoptosis by TUNEL. Cells were stained with TUNEL solution (red) and DAPI (blue). Adjustment of colors was applied in entire images to avoid reducing signals on merged images, and representative images are shown. Scale bar, 20 μm. The histogram is the quantification of TUNEL staining. The results are expressed as mean ± SEM. "Control" represents transfection with the empty plasmid; "Overexpress" represents transfection with the FABP4 overexpression vector; "siFABP4" represents transfection with siRNA; "NC" stands for interference negative control group.* p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001