Fig. 8
From: Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs
Functional verification of PLA2G4A in GCs. The concentration of arachidonic acid (AA) in cell culture medium was measured using a detection kit. A The ability of GCs to secrete AA. "Adult" represents GCs from adult ewes, and "Lamb-2D" represents GCs from lambs with 2-day FSH-treatment. B Effects of overexpression or interference of PLA2G4A in adult GCs on secretion of AA. C Effects of overexpression of PLA2G4A in lamb GCs on secretion of AA. "Control" represents the untreated cells; "Vector" represents the cells transfected with the empty plasmid, pIRES2-ZsGreen1;"Overexpress" represents the cells transfected with the PLA2G4A overexpression vector. "NC" stands for interference negative control group;"siPLA2G4A" represents transfection with siRNA. **** p < 0.0001