Fig. 9
From: Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs
Functional examination of GSTT2B in GCs. A-a The cell viability after overexpression or interference of GSTT2B in adult (A) and lamb (a) GCs. B-b The cellular ROS levels after overexpression or interference of GSTT2B in adult (B) and lamb (b) GCs. ROS levels were measured using a detection kit and were reflected by fluorescence intensity in each 1 × 104 cells. C–c Relative expression levels of antioxidant genes, SOD1, SOD2, CAT and GPX1 in adult (C) and lamb (c) GCs after overexpression or interference of GSTT2B. D Detection of cell apoptosis by TUNEL. Representative images of TUNEL (red) and DAPI (blue) stained cells are shown. Scale bar, 20 μm. The histogram is the quantification of TUNEL staining. The results are expressed as mean ± SEM. "control" represents the transfection with the empty plasmid, pIRES-puro3. "Overexpress" represents the transfection with GSTT2B overexpression vector. "NC" stands for interference negative control group; "siGSTT2B" represents the transfection with siRNA