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Table 1 Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

From: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

Name

Sequence (5′ → 3′)

Description

dTSO

AAGCAGTGGTATCAACGCAGAGTACGCGGG

TSO with typical DNA oligonucleotides

drTSO

AAGCAGTGGTATCAACGCAGAGTACGCr(GGG)

Chimeric DNA/RNA oligo sequence that carries 3 riboguanosines r(GGG) at its 3′ end

C3-TSO

AAGCAGTGGTATCAACGCAGAGTACGCr(GGG)-C3

Chimeric DNA/RNA oligo sequence that carries 3 riboguanosines r(GGG) at its 3′ end and a 3′ C3-spacer modification

F

AAGCAGTGGTATCAACGCAGAG

Forward universal primer designed to anneal at the TSO sequence for RACE reaction

Fnested

CAGTGGTATCAACGCAGAGTAC

Forward universal primer designed to anneal at the TSO sequence for nested RACE reaction