Table 4 The amino acid substitution scores for different kinds of codon substitutions
Codon Substitution | Random | Frameshift | Interchangeable | |
|---|---|---|---|---|
FF | RF | |||
Type of Codon Substitution | ||||
 All | 4096 | 256 | 256 | 256 |
 Unchanged (%) | 64 (1.6%) | 4 (1.6%) | 4 (1.6%) | 64 (25%) |
 Changed (%) | 4032 (98.4%) | 252 (98.4%) | 252 (98.4%) | 192 (75%) |
 SS (%) | 230 (5.6%) | 14 (5.5%) | 14 (5.5%) | 192 (75%) |
 NSS-Positive (%) | 859 (20.1%) | 76 (29.7%) | 76 (29.7%) | 40 (15.6%) |
 NSS-Negative (%) | 3007 (73.4%) | 166 (64.8%) | 166 (64.8%) | 24 (9.4%) |
Average Substitution Score | ||||
 BLOSSUM62 | −1.29 | −0.61 | −0.65 | 3.77 |
 PAM250 | −4.26 | −0.84 | −0.84 | 3.68 |
 GON250 | −10.81 | −1.78 | −1.78 | 35.60 |