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Table 4 The amino acid substitution scores for different kinds of codon substitutions

From: Frameshift and wild-type proteins are often highly similar because the genetic code and genomes were optimized for frameshift tolerance

Codon Substitution Random Frameshift Interchangeable
FF RF
Type of Codon Substitution
 All 4096 256 256 256
 Unchanged (%) 64 (1.6%) 4 (1.6%) 4 (1.6%) 64 (25%)
 Changed (%) 4032 (98.4%) 252 (98.4%) 252 (98.4%) 192 (75%)
 SS (%) 230 (5.6%) 14 (5.5%) 14 (5.5%) 192 (75%)
 NSS-Positive (%) 859 (20.1%) 76 (29.7%) 76 (29.7%) 40 (15.6%)
 NSS-Negative (%) 3007 (73.4%) 166 (64.8%) 166 (64.8%) 24 (9.4%)
Average Substitution Score
 BLOSSUM62 −1.29 −0.61 −0.65 3.77
 PAM250 −4.26 −0.84 −0.84 3.68
 GON250 −10.81 −1.78 −1.78 35.60
  1. SS/NSS Synonymous/nonsynonymous substitution, FF/RF Forward/reverse frameshift substitutions