Fig. 1

Starvation and refeeding affect zebrafish somatic growth as well as intestinal and environmental microbiome diversity. A Study design schematic. Cohoused adult siblings were divided into either control (fed) or experimental (starved) tanks. Samples were then taken from each tank on days 0, 1, 3, 7 and 21 post-starvation (dpS) as well as 1, 3, 7, and 21 days post-refeeding (dpR) for 16S rRNA gene sequencing. RNA-seq samples were taken at 3 dpS, 21 dpS, and 3 dpR. B Fed and starved zebrafish height at anterior of anal fin (HAA) in mm at corresponding timepoints. C Standard length in mm of starved and fed zebrafish. D Faith’s PD alpha diversity for fed and control zebrafish. Values are log transformed and normalized by the scores at day 0. E Weighted UniFrac distance between the gut and associated environment sample. Stars in panels B-E denote significant difference (p < 0.05 by Tukey’s HSD test)