Transcriptome analysis of blastoderms exposed to prolonged egg storage and short periods of incubation during egg storage

Table 2 Summary of Ingenuity Pathway Analysis results for C2 vs. CR comparison

Network Score	Top Functions
48	Organismal Survival
45	RNA Post-Transcriptional Modification, Small Molecule Biochemistry
41	RNA Post-Transcriptional Modification
Regulators in Network		Score	Targets	Functions
AREG, BAG1, MAP2K4		12.2	7	Cell survival, DNA repair
CXCL12, FSH, GPER1, GH, HGF, MAP2K1/2		5.9	15	mRNA expression, Immortalization
ANGPT2, PTPN3, Rb		3.1	13	Microtubule dynamics, Senescence of cells
Upstream Regulator	Fold	Molecule Type		Activation z-score	P Value
MYC	−1.564	transcription regulator		−2.606	1.31E-12
CXCL12	−1.671	cytokine		−2.523	4.37E-02
CCND1	−1.901	transcription regulator		−2.274	1.18E-05
S100A6	2.441	transporter		−2.236	1.33E-02
SOX17	2.573	transcription regulator		2.393	1.22E-03
Master Regulator	Fold	Molecule Type		Activation z-score	P Value
CCNF	−1.866	other		−3.43	3.50E-03
KDM5B	1.588	transcription regulator		−2.949	7.00E-04
TFDP2	−1.877	transcription regulator		−2.832	5.00E-04
MNAT1	−1.798	other		−2.554	1.80E-02
TLK2	−1.538	kinase		−2.236	2.22E-02

The top three networks (network score > 40) and top three regulator effect networks (consistency score > 0, target number > 3, |z-score| > 2, p-value < 0.05) are shown. The top five activated (z-score > 2) and inhibited (z-score < − 2) upstream regulators and master regulators in causal networks are shown (|fold change| > 1.5, |z-score| > 2, p-value < 0.05). Only upstream regulators and master regulators exhibiting differential expression between C2 and CR groups are displayed

ISSN: 1471-2164