Transcriptome analysis of blastoderms exposed to prolonged egg storage and short periods of incubation during egg storage

Table 4 Summary of Ingenuity Pathway Analysis results for NSR vs. SR comparison

Network Score	Top Functions
43	Cell Death and Survival, Cellular Function and Maintenance, Embryonic Development
43	Carbohydrate Metabolism, Nucleic Acid Metabolism
43	Cell Cycle, Cell Morphology, DNA Replication, Recombination, and Repair
Regulators in Network	Score	Targets	Functions
MXD1, PDGF BB	3.3	5	Cell viability, Migration of cells, Synthesis of protein, mRNA stabilization, RNA repression
MAP2K4, MXD1, PDGF BB	2.5	13	Expression of mRNA, rRNA modification
PCGEM1	2	9	Cell proliferation
Upstream Regulator	Fold	Molecule Type	Activation z-score	P Value
TWNK	−1.604	enzyme	−2.72	5.77E-07
CCNK	1.53	kinase	−2.433	0.0373
H2AX	1.84	transcription regulator	−2.418	0.00207
SAFB	−1.876	other	−2.333	0.0301
Master Regulator	Fold	Molecule Type	Activation z-score	P Value
PA2G4	−1.52	transcription regulator	−4.518	0.0001
PDCD4	1.749	other	−4.5	0.0001
CHAF1A	−1.649	other	−4.205	0.0001
HMGA1	−1.608	transcription regulator	−4.106	0.0001
DNAJB1	1.756	transcription regulator	4.643	0.0001
TADA3	−2.638	transcription regulator	4.687	0.0001
BARD1	2.675	transcription regulator	4.692	0.0001
CBL	2.257	transcription regulator	4.739	0.0026
TSC22D1	2.081	transcription regulator	4.826	0.0001

The top three networks (network score > 40) and top three regulator effect networks (consistency score > 0, target number > 3, |z-score| > 2, p-value < 0.05) are shown. The top five activated (z-score > 2) and inhibited (z-score < −2) upstream regulators and master regulators in causal networks are shown (|fold change| > 1.5, |z-score| > 2, p-value < 0.05). Only upstream regulators and master regulators exhibiting differential expression between NSR and SR groups are displayed

ISSN: 1471-2164