Fig. 3

Structural variations within 5S rDNA clusters between C. elegans N2a and CB4856 strains. a INDELs identified with CB4856 ONT reads within the 5S rDNA unit as in Fig. 1a. Cross-validated INDELs used in the subsequent analysis are indicated with black circles (see Methods). b SNPs in the 5S rDNA are identified with existing NGS data as in Fig. 1b. SNPs present or absent in new rDNA variants are colored in red and black, respectively. c Overview of the structures of 5S rDNA clusters between N2a and CB4856 as shown in Fig. 1d. Note the differences between the two, including lack of unit 1.1 (red) predominantly seen in N2a, whereas the unit (cel-5S unit 1.17 (see Table 2 & S5)) is unique to and predominantly seen in CB4856. d Structure of the C. elegans CB4856 5S rDNA cluster. The Repeat 1a and 1b are shown as in Fig. 1d