Fig. 2
From: Elevated ASCL1 activity creates de novo regulatory elements associated with neuronal differentiation
Dephosphorylation of ASCL1 further enhances its genome-wide binding. a Western blot showing overexpression of WT and S-A ASCL1 in two pairs of unique clones after 24 h induction with 1 μg/ml doxycycline, α-tubulin loading control. Endo. = endogenous. Full-length blot is presented in Supplementary Fig. 6. b Average profile (top) and aligned heatmap (bottom) showing WT and S-A ASCL1 binding (n = 8) at common, S-A gained, and S-A lost peaks, alongside endogenous histone modification data from SH-SY5Y cells (H3K27ac, H3K4me1, H3K4me3, H3K27me3) or LAN6 cells (H3K9me3) [30, 31]. Data shown ± 3 kb from the peak centre. c Stacked barplot showing the distribution of ASCL1 common, S-A gained, and S-A lost peaks in genomic features. Actual numbers of peaks shown in each segment. d Top enriched E-box motif identified in common, S-A gained, and S-A lost ASCL1 peaks, against a background of random sequences matched for GC content