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Fig. 4 | BMC Genomics

Fig. 4

From: Identification of circRNA-associated ceRNA networks using longissimus thoracis of pigs of different breeds and growth stages

Fig. 4

Verification of circRNA structure (A) Identification of circRNA candidates using divergent and convergent primers. Divergent primers amplified circRNA targets in cDNA but not genomic DNA, and convergent primers amplified targets in both. (B) RT-qPCR to assess circRNA abundance and mRNA expression of host genes. GAPDH served as the reference gene. Note: Values are mean ± S.E.M. Data represent six independent assessment methods. Student’s t-test was used to compare expression levels or values among different groups. *P < 0.05; **P < 0.01

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