Fig. 5
Inhibition of C2C12 cell proliferation by circKANSL1L (A) Relative expression of circKANSL1L and mRNA-KANSL1L in C2C12 cells following circKANSL1L overexpression or inhibition. GAPDH served as the reference gene. (B) Relative RNA and (c, above panel) protein levels of cell proliferation-related genes in C2C12 cells following circKANSL1L overexpression or inhibition. (C, below panel) PCNA, CyclinD1, and CyclinE band intensities quantified by Image J and normalized against internal reference Tubulin. (D) Growth curve of C2C12 cells following circKANSL1L overexpression and inhibition. (E) Cell cycle analysis of C2C12 cells following circKANSL1L overexpression or inhibition. Note: Values are mean ± S.E.M. Data represent six independent assessment methods. Student’s t-test was used to compare expression levels or values among different groups. *P < 0.05; **P < 0.01